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To characterize and explore the endogenetic fungi of Chinese Cordyceps from Tibetan plateau for their bioactive potential, more than 30 kinds of endogenetic fungi were routinely isolated from Chinese Cordyceps. Six selected isolates were further investigated on the mycelial growth rate, morphology and molecular characteristics and the content of nucleosides in the mycelial fermentation products. The results showed that all the six strains could grow well in the ordinary media. Analysis of their molecular and morphological characteristics confirmed that the six strains belonged to the genus of Fusarium, Mucor and Mortierella, respectively. The nucleosides including uridine, guanosine, adenosine and cordycepin had been detected in the mycelial fermentation products of all the 6 strains. The strain NS2-B1 was the best one in all strains, and could produce the most nucleosides with a yield of 1284.47±26.05 μg/g. Meanwhile the strain NS2-B1 could produce more adenosine and cordycepin (418.72±2.38 μg/g and 179.14±0.57 μg/g), which was significantly higher than that of Chinese Cordyceps (18.47±0.31 μg/g and 90.43±1.46 μg/g; p<0.01). This study proved that the selected strain NS2-B1 had a potential application value. © 2017 Friends Science Publishers

Identification and Bioactive Potential of Endogenetic Fungi Isolated from Medicinal Caterpillar Fungus Ophiocordyceps sinensis from Tibetan Plateau