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The maternal Immunoglobulin G (IgG) antibodies transferred during the intrauterine life represents the main newborn immune protection against several intestinal and respiratory infection. However, there is little information about the reactivity and function of IgG from cord blood against newborn oral colonization. The aims this study was to evaluate the presence of IgG against Streptococcus mitis, Streptococcus mutans and theirs mains virulence antigens (GbpB, Ag I/II and Gtf) in peripheral blood (PB) samples from mothers with S. mutans or not in the saliva (SA) and compare with umbilical cord blood (CB). PB and SA samples were obtained from healthy women in the Hospital admission and CB was collected after delivery. The specificity of IgG against the bacterial extracts was determined by Western blot. The genetic material detection of S. mutans in the salivas was realized by the quantitative polymerase chain reaction with specific primers. The results showed that the minority of blood samples showed IgG against S. mitis. On the other hand, the majority of samples exhibited IgG anti S. mutans. The number of reactive bands to S. mutans was significantly higher than against S. mitis in both blood samples (p 0.05) in the PB from mothers colonized or not by S. mutans. In opposite, in CB sample, it was more frequent to find samples with IgG anti-S. mutans and GbpB in the salivas from mothers with detectable S. mutans (p<0.05). In conclusion, the blood samples possessed IgG-anti S. mutans and theirs virulence antigens; mainly IgG anti-GbpB. The lack of IgG against S. mitis transferred by CB can be related to the early S. mitis colonization in the first months of age. The similarity of bands recognized by IgG against S. mutans antigens detected in PB and CB is suggestive that maternal antibodies can be transferred to the fetus. Keywords: Streptococcus mutans. Saliva. Fetal Blood. Immunoglobulin G Resumo Os anticorpos Imunoglobulina G (IgG) transferidos durante a vida intrauterina representam a principal protecao imunologica do recem-nascido contra diversas infeccoes intestinais e respiratorias. No entanto, poucas informacoes existem sobre a reatividade e funcao de IgG do sangue do cordao contra a colonizacao oral de recem-nascidos. Os objetivos deste estudo foram avaliar a presenca de IgG contra Streptococcus mitis, Streptococcus mutans e seus principais antigenos de virulencia (GbpB, Ag I / II e Gtf) em amostras de sangue periferico (SP) de maes com S. mutans detectavel ou nao na saliva (SA) e comparar com sangue do cordao umbilical (SC). Amostras de SP e SA foram obtidas de mulheres saudaveis na admissao do hospital e CB foi coletado apos o parto. A especificidade de IgG contra os extratos bacterianos foi determinada por Western blot. A deteccao do material genetico de S. mutans nas salivas foi realizada pela reacao em cadeia da polimerase quantitativa com primers especificos. Os resultados mostraram que a minoria das amostras de sangue apresentou IgG contra S. mitis. Por outro lado, a maioria das amostras exibiu IgG anti S. mutans. O numero de bandas reativas para S. mutans foi significativamente maior do que contra S. mitis em ambas as amostras de sangue (p 0,05) no SP de maes colonizadas ou nao por S. mutans. Por outro lado, nas amostras SC, foi mais frequente encontrar amostras com IgG anti-S.mutans e GbpB em salivas de maes com S. mutans detectaveis (p <0,05). Em conclusao, as amostras de sangue possuiam IgG-anti S. mutans e seus antigenos de virulencia; principalmente IgG anti-GbpB. A falta de IgG contra S. mitis transferida no SC pode estar relacionado a colonizacao precoce de S. mitis nos primeiros meses de idade. A semelhanca de bandas reconhecidas por IgG contra antigenos de S. mutans detectados em SP e SC e sugestiva de que anticorpos maternos podem ser transferidos para o feto. Palavras-chave: Streptococcus mutans. Saliva. Sangue Fetal. Imunoglobulina G

Detection of Immunoglobulin G Against Oral Streptococci in Peripheral and Umbilical Cord Blood