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. In this study, the cytotoxic effects of Z. multifloraessential oilwere investigated on human adenocarcinoma gastric cell line (AGS) and human fibroblast cells. The AGS cells were seeded in 96-well culture plates in the presence and absence of different concentrations of Z. multifloraessential oil(0, 6.25, 12.5, 25, 50, 100, 200 and 400 µg/ml)for 24, 48 and 72 hours using MTT assay. Results showed thatessential oil of Z. multiflorashowed strong inhibitory effects on AGS cells when compared to control. The cytotoxicity effect after 24h incubation was greater than 48 and 72h and the highest cytotoxicity of Z. multifloraessential oil was related to 400 μg/ml (90.01%) . Also results showed that more effect of Z. multifloraessenceon the growth inhibitory of normal fibroblast cell was observed at 100 μg/ml after 48h (98.82%).

Cytotoxic effect of Zataria multiflora on gastric cancer cell line (AGS) and normal fibroblast cells