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Objectives: High-frequency skin ultrasound (HFSU) allows for an extremely detailed assessment of the skin layers (epidermis, dermis, and hypodermis), as well as skin appendages (hair follicles, glands, and vessels). The aim of the study was to evaluate vulvar structures using HFSU and its utility as a diagnostic tool. Material and methods: The study group consisted of 50 women (22 preand 28 post-menopausal), aged 1881 years, without clinical symptoms of vulvar pathology. The following regions were assessed: mons pubis, labia majora, labia minora, external urethra orifice, and posterior commissure. Statistical analysis of epidermis and dermis for mons pubis, labia majora and minora was performed. Results: Comparison of the epidermis (EP) and dermis (DER) of mons pubis (MP), labia majora (VLM), labia minora (VLm) between the preand postmenopausal groups showed no statistically significant differences. Further comparison of EP and DER deemed the following results: for the premenopausal group EP MP vs. EP VLM (p=0.0884) and DER MP vs. DER VLm (p=0.8874), for the postmenopausal group EP MP vs. EP VLM (p=0.5755) and DER MP vs. DER VLm (p=0.3606), and without grouping EP MP vs. EP VLM (p=0.0988) and DER MP vs. DER VLm (p=0,5092), and showed no statistical differences. As for rest of the measured parameters, the p-value was <0.05. Conclusions: HFSU allows for precise imaging and measurement of vulvar epidermis and dermis.

Feasibility of using high-frequency skin ultrasound (HFSU) in vulvar skin assessment – initial report with the description of HFSU anatomy.