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These research was a descriptive study that aims to describe the activity of phytase from endophytes bacteria isolated from Zea Mays L. Has been isolated and identified, four endophytes bacteria from Zea Mays L. ie. Burkholderia sp. strain HF.7 derived from root, Enterobacter cloacae strain HF.8 derived from stem, Enterobacter ludwigii strain HF.16 derived from leaves, and Pantoea stewartii strain HF.28 from seeds. The activity of phytase was known by measuring the absorbance value of the crude extract of phytase at λ = 700 nm which then analyzed the amount of phosphate content formed (FTU/mL) in solution of crude extract of enzyme by substitution of absorbance value using linear regression equation of standard phosphate curve. The phytase activity of each endophytic bacteria was as follows: 7.83 FTU/mL; 5.24 FTU/mL; 6.34 FTU/mL; and 4.93 FTU/mL. Fourth endophytic bacteria of Zea Mays L. which indicates a relatively high phytase activity, was an opportunity to use it as a feed additive in order to improve the quality of poultry feed.

Phytase Activity of Four Endophytes Bacteria from Zea Mays L.