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Free radical of a species having unpaired free electrons can cause damage to the cells and trigger various diseases, It can be reduced by antioxidant compounds. This study aims to determine the content of secondary metabolites and antioxidant activity stem bark extract of Xylocarpus granatum. Stem bark of Xylocarpus granatum was macerated using etanol to obtained the extract, The extract will be fractionated using solvent with different pollarity. Phytochemical analyses to identification some secondary metabolite used some reagent. Antioxidant activity evaluated by using 2, 2diphenyl-1-picryl-hydrazyl-hydrate assay The results showed Stem bark Xylocarpus granatum has secondary metabolite compound type Alkaloid, Flavanoid, steroids, polyphenols, and tannin. the antioxidant activity through DPPH radical scavenging showed IC50 value on 31 μg / mL methanol extract, ethyl acetate extract 44.35 μg / mL, n-hexane extract 159.82 μg / mL and at fraction of nhexane, ethyl acetate, and water respectively 87.6 μg / mL, 49.31 μg / mL, 41.1 μg / mL and ascorbic acid used as a standard of 8.13 μg / mL. finally stem bark extract of Xylocarpus granatum has the ability to reduce the radical DPPH so potentially as a natural antioxidant.

Phytochemical Screening and Antioxidant Activity Stem Bark Extract of Xylocarpus Granatum from Nothern Buton