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| The 96 h LC50 and lethal concentration of Fe+Ni+Pb+Zn mixture for the fish, Cirrhina mrigala was determined in this research work. The fish were exposed to each of the 19 different concentrations (5-90 mg/L) of Fe+Zn+Pb+Ni mixture to determine 96 h LC50 and lethal concentration. During acute toxicity test, behavioral changes and catalase activity in liver, kidney, gills, brain and muscle of the fish were also studied. The mean 96 h LC50 and lethal value for C. mrigala was determined as 47.56±0.04 and 93.89±0.07 mgL-1, respectively. The selected concentration of Fe+Ni+Pb+Zn concentrations showed strong and direct relationship with, fin movement followed by mucous secretion, hyperactivity and swimming rate for C. mrigala with R2 value of 0.858, 0.853, 0.216 and 0.005, respectively. The Fe+Ni+Pb+Zn mixture exposure at 96 h LC50 concentration caused significant decrease of catalase activity in liver, kidney, gills, muscle and brain of C. mrigala as compared to control. This study suggests that the behavioral and biochemical parameters can be used as a valuable bio-marker to identify the toxic effects of pollutants in aquatic systems. Article History Received: February 16, 2018 Revised: April 28, 2018 Accepted: May 01, 2018 Published: June 20, 2018 Authors’ Contributions HM executed the research. SA supervised and guided in planning the research work. SN did statistical analysis. SA assisted in compiling the data. MB and WH helped in lab work. SP and LS helped in writing the manuscript.

Comparative Assessment of the Acute Toxicity, Behavior and Catalase Activity in Cirrhina mrigala Exposed to Fe+Ni+Pb+Zn Mixture