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| Anaplasmosis is disease of livestock having pandemic in nature. In the present study the specificity and sensitivity of i-ELISA and RT PCR was investigated in the samples collected from District Peshawar and Lakki Marwat. A total of 900 samples were collected from three species, Cattle, Buffalo and Sheep from two transects Peshawar and Lakki Marwat for determination of rate of Anaplasma marginale through i-ELISA and RT PCR. Overall rate for Anaplasmosis was 37.7% (339/900) for all three techniques, whereas techniques wise the rate of Anaplasma marginale was 28.7% and 34.8 % through i-ELISA and Real-time PCR, respectively. Majority of the Cattle (41.6 %) were found positive for A. marginale as compared to 23.7% and 16.2% in Sheep and Buffalo respectively. Transects were significantly different at P <0.05 in term of rate of A. marginale. Transect wise distribution of anaplasmosis was higher in Lakki Marwat (41.5%) as compared to Peshawar (30%). Gender wise rate was higher in female through i-ELISA (57.96%) and RT PCR (64.33%) as compared to male individuals having 16.27% and 23.25% rate, respectively. A significant difference was found in seasonality of tick infestation, with the high infestations in summer as compare to winter.

south asian journal of life sciences

Comparative Analysis of Indirect ELISA and Real Time PCR for the Detection of Anaplasma Marginale in Buffalo, Cattle and Sheep in District Peshawar and Lakki Marwat, Pakistan