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| To find the suitable cryoprotectants for cryopreservation of ram semen, two cryoprotectants ethylene glycol and glycerol were added to semen at different concentrations and glycerol (5%) was added at different temperatures to Magra ram semen. Ejaculates (n=36) collected from adult Magra rams (n=6) during the breeding season, were subjected to routine evaluation of seminal parameters and were cryopreserved by adding either glycerol (5%) added extender at two temperatures of semen (either 5oC or 30oC) or adding either glycerol or ethylene glycol at different concentrations (2%, 3%, 4%, 5%, 6% or 7%). The post-thaw motility of cryopreserved semen was evaluated 7 days later by thawing at 37oC for 30 seconds. The overall mean sperm concentration, initial individual motility and live percentage of spermatozoa observed were 2999.33 ± 3.51(million/ml), 80.05 ± 0.20 % and 85.30 ± 0.22 %, respectively. The postthaw live percentage of spermatozoa, in semen was significantly higher (P 0.01) when the glycerol added extender was mixed at 30oC compared to cooled semen at 5oC. The post-thaw motility of Magra ram semen was highest 50±0.74 % at 6% glycerol concentration whereas it was 44.18±1.17% in ethylene glycol at 2%. At concentrations of 3% to 7% addition of glycerol resulted in significantly higher post-thaw motility of semen compared to ethylene glycol. It was concluded that it is better to supplement glycerol added (6%) extenders in ram semen at 30oC and ethylene glycol above 2% results in a reduction of postthaw motility and percent live sperms.

Comparative Effect of Different Concentrations of Glycerol and Ethylene Glycol and Temperature on Cryopreservation of Ram Semen