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| Of the aim of the current study is to study the cytotoxic effect of Zearalenone and T-2 toxin on the sperm of bulls and stallions following the Kharkov technology of sperm obtaining and cryopreservation. Invitro toxicity effect of various concentrations of Zearalenone and T-2 toxin (0.5 mM to 0.01 mM) on the membrane integrity, as well as on the biological indicators of the spermatozoa of bulls and has been shown. Our results revealed that after adding 0.5 mM and 0.25 mM of Zearalenone, the biological activity of native sperm cells after one hour did not change, and after thawing, it decreased by 19.4 % (P < 0.01) and 12.2 % (P < 0.05); respectively, compared to non-treated negative control. On the other hand, the T-2 toxin at the concentration of 0.5  mM and 0.25 mM reduced the biological activity of native sperm cells after one hour of incubation by 10.14 % (P < 0.01) and 8.03 % (P < 0.05), respectively and their activity after thawing decreased by 60.28 % (P < 0.001) and 52.7 % (P < 0.001); respectively, compared to non-treated negative control. In this study, the lowest concentration that gave a veracious effect on the characteristics of the sperm was 0.01 mM of Zearalenone and the T-2 toxin, and after thawing only a slight decrease in the activity and survival rate of sperm that was 2.3 and 6.5 %, respectively. While at the dosage of 0.01 mM of Zearalenone and T-2 toxin, a veraciously significant decrease in biological survival rate and the absolute survival indicator of native sperm by 8.7 and 4.3 % (P < 0.05), respectively. The cytotoxic effect of Zearalenone and T-2 toxin on the sperm of bulls and stallions in vitro after cryopreservation has been studied in vitro after cryopreservation. It has been found that the minimum cytotoxic dosage of Zearalenone and T-2 toxin during the cryopreservation was 0.01 mM. Likewise, the species differences have been shown in the sensitivity of the spermatozoa of bulls and stallions for the cytotoxic effect of Zearalenone and T-2 toxin during cryopreservation, which is evidence to the fact that complex addition of Zearalenone and T-2 toxin to the diluent has lower toxicity to the plasmalemma, acrosome, and the biological parameters of the spermatozoa of bulls than adding T-2 toxin alone, which is not observed in the sperm of stallions.

Effect of Mycotoxins on the Spermatozoa and Embryos of Animals