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| The split-conjugated vaccine against brucellosis of animals was tested as a provocateur in order to identify sick (latent) animals in both brucellosis safe and brucellosis poor farms. After administration of the test vaccine, antibodies remained in the blood of healthy animals for up to 2.5 months, then disappeared, and antibodies remained in the blood of sick animals after 3.5, 4.5 and 7.5 months and gradually disappearing over time. The split-conjugated animal brucellosis vaccine has a “provocative effect”, exacerbates the infectious process and thereby makes it possible to identify sick animals. We recommend the use of a split-conjugated animal brucellosis vaccine in brucellosis-poor households in order to detect latent animals and create humoral immunity in animals while maintaining antibodies in the blood of animals for 2.5 months. In the case of small detection of animals with brucellosis in the farm (from 1 to 5 animals), we recommend using the vaccine to detect animals with brucellosis and to create immunity in healthy animals twice with an interval of 3 months. If a large number of animals with brucellosis in the farm (from 6 to 20 and above goals) are detected, we recommend using the vaccine once after eliminating the sick livestock, followed by the use of the vaccine from the Brucella abortus 82 strain, which must be vaccinated after passing calving (May July). The split-conjugated animal brucellosis vaccine can be used on both non-pregnant and pregnant cows, as it does not cause abortion.

The Application of a Split-Conjugated Anti-Brucellosis Vaccine as a “Provocing Factor” for Identification of Brucellosis in Animals