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| Background and Aims: To determine the possible stress factors affecting normal physiological level of gamma interferon in calves. Methods: Forty two healthy calves with age rang (1-6 months ) were included. Five milliliters of venous blood were collected and store at -20 co for evaluation of gamma interferon (INFγ). Complete investigation about the age, gender, source of feed, source of water, health status of breeding environment and season was recorded. results: The mean INFγ concentration was (126.2714± 14.43685 pg/ml). Significant difference was reported between age groups according to INFγ level (p value =0.000). INFγ level positively correlated with calves age group (1-2) month (p value =0.043); female calves (p value =0.014),while significant negative correlation was reported between the male calves and INFγ level (p value =0.032). INFγ level positively correlated with drinking of tap water (p value =.003) and milk feeding (p value =0.050). INFγ level positively Inversely correlated with grass feeding (p value =0.050); breeding of calves in poor health status of environment (p value =0.0050).Although significant fluctuation in INFγ level was obvious throughout the year , no significant correlation between season and mean INFγ level. conclusions: Normal physiological level of INFγ in calves positively increased during 1-2 months after birth, positively correlated with gender mainly females; drinking of tap water; milk feeding. Normal physiological level of INFγ in calves inversely correlated with males; grass feeding; breeding of calves in poor health status of environment. Normal INFγ level in calves not affected by season.

Evaluation of Possible Stress Factors Affecting Physiological Level of Gamma Interferon During First Six Months of Life in Healthy Calves