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K e y w o r d s : chromosome, karyotype, Saxifraga manchuriensis, Russian Far East Р Е З Ю М Е Фукуда Т., Колдаева М.Н., Икеда Х. Кариологичес кое исследование Micranthes manchuriensis (Engl.) Gornall & H. Oh ba (Saxifragaceae) из Приморского края, Россия. Кариологическое­ ис­ сле­ до­вание­ Micranthes man chu riensis­(Engl.)­Gornall­&­H.­Ohba­(Saxifragaceae)­из­При­морского­края,­ Россия­показало,­что­число­хро­мо­сом­у­исследованных­растений­2n­=­30.­ Кариограмма­M. manchuriensis­описывается­формулой­13­m­+­2­sm,­с­одной­ парой­сателлитных­хромосом. К л ю ч е в ы е с л о в а : хромосома,­кариотип,­Saxifraga manchuriensis,­российский­ Дальний­Восток Tomoko Fukuda 1 * e­mail: fukudatomoko@gmail.com Marina N. Koldaeva 2 e­mail: mnkoldaeva@mail.ru Hiroshi Ikeda 3 e­mail: h_ikeda@um.u­tokyo.ac.jp 1 Department of Botany, National Museum of Nature and Science, Ibaraki 305­0005 Japan 2 Botanical Garden­Institute FEB RAS, Vladivostok 690024, Russia 3 The University Museum, The University of Tokyo, Tokyo, 111­0033, Japan * author for correspondence Manuscript received: 10.10.2015 Review completed: 17.02.2016 Accepted for publication: 01.03.2016 Published online: 06.05.2016 Tomoko Fukuda1*, Marina N. Koldaeva2 & Hiroshi Ikeda3 A karyological study of Micranthes manchuriensis (Engl.) Gornall & H. Ohba (Saxifragaceae) from Primorsky Krai, Russia Micranthes manchuriensis (Engl.) Gornall & H. Ohba (Saxi­ fragaceae) is a perennial herb, distributed in Russia (Primorsky Krai), northern China, and the Korean peninsula (Zhmylev 1996, Barkalov et al. 2011). It is similar to M. nelsoniana (D. Don) Small in having round to reniform leaves with triangular­deltoid teeth,­a­paniculate­inflorescence,­and­white­petals.­It­grows­on­ the banks of streams and on moist riverine rocks. Engler (1872) treated it as a variety of Saxifraga punctata L. (= M. nelsoniana). However, it differs from M. nelsoniana in having thick leaves, flowering­stems­with­dense­septate­hairs,­and­congested,­almost­ capitate­ inflorescences­ (Siplivinsky­ 1976,­ Voroshilov­ 1982,­ Charkevicz 1989, Webb & Gornall 1989, Zhmylev 1996, Bar­ ka lov et al. 2011, Akiyama et al. 2012). The only chromosome number of Micranthes manchuriensis was reported by Sokolovs­ kaya (1966) as 2n = 30–32. In June 2015, we collected Micranthes manchuriensis from two localities in Primorsky Krai, Russia (Table 1), and a karyologi­ cal­study­was­performed­to­confirm­the­chromosome­number. Root tips were pretreated in 0.002M 8­hydroxyquinoline solution­ for­ 12­hours­ at­ 5°С,­fixed­with­Farmer’s­ solution­ (glacial acetic acid: 99 % ethanol = 1:3) and kept in a re fri­ ge­rator­at­5°С­for­more­than­10­hours.­The­root­tips­were­ then­macerated­in­1N­HCl­for­10­minutes­at­60°С,­stained­ with 1 % aceto­orcein for 10–20 minutes, and squashed. Mitotic metaphase chromosomes were observed for 8 in di­ viduals. The karyotype was analyzed for one individual (Fu­ kuda with Koldaeva, No. 2015­BG­1). Voucher specimens are deposited in the Botanical Garden­Institute of the Far Eastern Branch, Russian Academy of Sciences, Vladivostok (VBGI). Locality Latitude & longitude 2n Voucher Lazovsky District, under waterfall of Elomovsky River 43°14'45"N, 133°43'05"E 30 T. Fukuda with V. Bakalin 2015­Elw­2 30 T. Fukuda with V. Bakalin 2015­Elw­6 30 T. Fukuda with V. Bakalin 2015­Elw­8 Lazovsky District, along Elomovsky River, ca. 4 km lower stream from waterfall 43°13'45"N, 133°45'34"E 30 T. Fukuda with V. Bakalin 2015­Elm­1­11 30 T. Fukuda with V. Bakalin 2015­Elm­2­10 30 T. Fukuda with V. Bakalin 2015­Elm­2­13 Vladivostok, Botanical Garden­Institute FEB RAS 43°13'23"N, 131°59'39"E 30 T. Fukuda with M. Koldaeva 2015­BG­1 30 T. Fukuda with M. Koldaeva 2015­BG­2 Table 1 Locality, somatic chromosome number, and voucher specimen of Micranthes manchuriensis used in this study