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Low yields and productivity in fermentation industry usually due to poor quality of seed culture (inoculum). As the first stage in the fermentation process, inoculum consistency in terms of size and quality is clearly important for high yield of enzymes production. Selection of microorganism also plays an important role in improving the productivity. Fungi type of white rot basidiomycetes species is a well-known cellulolytic enzymes producer and capable to degrade many types of lignocellulosic biomass. Cellulolytic enzymes produced from white rot fungi, Pycnoporus sanguineus (PS) were investigated. The PS cultured on different agar media and parameter conditions of seed culture preparation in liquid medium broth were compared. Seed culture conditions of PS were influenced by many factors such as type of medium used for fungal growth, temperature, incubation time in liquid medium and subculture time on agar medium. PS was full-grown on potato dextrose agar (PDA) on day 5 compared to malt extract agar (MEA) on day 7. Seed culture conditions was determined using standard liquid medium, potato dextrose broth (PDB) at constant temperature (30 C), agitation speed (150 rpm) and pH (4.8) for duration of seven days using two different subculture of PS that were grown for five and seven days. It was found that the five days’ subculture of PS shown 3.93% higher cell dry weight of fungal biomass (3.44 g/L on day 5) and higher cellulolytic enzymes activity; 95.49% (FPase), 3.14% (CMCase), 7.71% (exoglucanases) and 14.93% (xylanases). Maximum cellulolytic enzymes were found after 48 hours’ incubation with filter paper activity (FPase) of 1.79 U/mL, carboxymethyl cellulase activity (CMCase) of 3.36 U/mL, exoglucanase activity (Avicelase) of 0.59 U/mL and xylanase enzymes activity of 0.66 U/mL. Reducing sugars concentration decreased from day 1 to 7 due to consumption of sugars for fungal growth. Seed culture conditions were strongly influenced by subculture and incubation time in liquid medium to produce high yield of cellulolytic enzymes from PS.

SEED CULTURE CONDITIONS FOR HIGH YIELD OF CELLULOLYTIC ENZYMES PRODUCTION FROM Pycnoporus sanguineus