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This study investigates the production of acetone, butanol and ethanol (ABE) in sterilized sludge of palm oil mill effluent (POME) with the addition of glucose by Clostridium acetobutylicum NCIMB 619. Glucose concentrations of 5 g/L, 10 g/L and 15 g/L were used with volume percentage of 3% (v/v) and 5% (v/v) for each concentration. The highest ABE production of 9.89 g/L was observed with the addition of 10 g/L glucose with volume percentage of 5% (v/v) compared to 0.49 g/L in the control batch, with highest ABE productivity and yield, YP/S of 0.14 g/L/h and 1.36 g ABE/g substrate, respectively. Providing culture with 15 g/L glucose with volume percentage of 5% (v/v) was found to reduce the ABE production with lower productivity and ABE yield. The lower production at higher sugar level might be due to sugar inhibition that occurred during the process.

THE EFFECT OF GLUCOSE ADDITION IN ACETONE-BUTANOL-ETHANOL FERMENTATION FROM PALM OIL MILL EFFLUENT BY Clostridium Acetobutylicum NCIMB 619