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This study was conducted to evaluate the nitrogen use efficiency of rice mutant lines of MR219, viz. MR219-4 and MR219-9, developed under rice radiation mutagenesis programme for adaptability to aerobic conditions, grown under conditions of varying water potentials and nitrogen levels in a shade house. Direct 15 N isotopic tracer method was used in this study, whereby the 15 N labeled urea fertilizer was utilized as a tracer for nitrogen nutrient uptake by the test crops. 15 N isotope presence in the samples was determined by using emission spectrometer analysis and percentage of total nitrogen was determined by using Kjeldahl method. 15 N atom access values of the samples were to determine the efficiency of N use by the mutant varieties. In this work, the data of yield components, viz. grain yield, plant biomass, plant height and 1000 grain weight, and nitrogen components viz, total N, % nitrogen derived from labeled fertilizer (% Ndff), % nitrogen derived from soil (% Ndfs), nitrogen uptake, fertilizer nitrogen use and nitrogen use efficiency were obtained. Results from this study showed a significant difference to the nitrogen component and yield when there is a change in the rate of fertilizer and water potential. However, varietal factor does not show any significant differences.

the malaysian journal of analytical sciences

NITROGEN USE EFFICIENCY IN MR219-4 AND MR219-9 RICE MUTANT LINES UNDER DIFFERENT WATER POTENTIALS AND NITROGEN LEVELS USING 15N ISOTOPIC TRACER TECHNIQUE