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The ability of wild-type strain Cupriavidus eutrophus B10646 to synthesize copolymers containing 3-hydroxy-4-methylvalerate (3H4MV) (P(3HB/3HV/3H4MV)) was studied. The possibility of synthesis of terpolymers containing 3HB, 3HV and 3H4MV monomers using glucose, or butyric acid and co-substrate was shown. A family of copolymers with different content of 3H4MV monomer was synthesized. The highest content of 3H4MV was 7.7 mol. %. Increase of 3HV and 3H4MV content in copolymers caused the reduction of crystallinity degree and of molecular weight, and changes of thermal characteristics of copolymers.

Biosynthesis and Properties of PHA Containing Monomers 3-Hydroxy-4-Methylvalerate