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Unique physicochemical properties of nanoparticles make them a novel candidate in agriculture and related areas. In the present work, we have studied the effect of casein stabilized metal and metal oxide nanoparticles, viz. AgNPs, AuNPs, and CuONPs, on in vitro propagation of Scoparia dulcis L. We have examined the effect of these nanoparticles on the callus induction, shoot regeneration, and root regeneration capacity. It was observed that Ag, Au, and CuO nanoparticles had interacted with the tissues differently and produced calluses with difference in color, nature, and texture. Nanoparticles also affected shoot regeneration, root regeneration, phenolic compound production, and chlorophyll content. Explants showed a very good response when treated with CuONPs. The formed callus underwent shoot and root regeneration without changing the medium

Casein Stabilized Metal and Metal Oxide Nanoparticles for the Efficient In Vitro Culturing of Scoparia dulcis L.