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On the basis of experimentally achieved and developed technical and technological parameters of biosynthesis processes the baseline data were obtained and the project was worked out for organization of pilot production of degradable polyhydroxyalkanoates (PHAs). The pilot line for production of polyhydroxyalkanoates was designed, equipped and commissioned, including the “Bioengineering” fermentation line (Switzerland), as well as the blocks for preparation of inoculum and culture media, isolation and purification of polymers. The pilot production was commissioned. When the initial concentration of inoculum was (10 ± 2) g/l and the cultivation duration was 65 h, the final concentrations of cells biomass in the culture of Cupriavidus eutrophus В10646 and polymer were (110±10) g/l and (85±5)%, with the process productivity by biomass and PHA of 1.7 and 1.4 g/l∙h, respectively, which is twice as high as the previously achieved results in the culture of Ralstonia eutrophus B5786 on fructose. Scaling the technology made it possible to obtain the experimental batches of polymers in the amounts needed for conducting a complex of prescribed research, development and standardization of specialized polymer products.

Scaling of Biodegradable Polyhydroxyalkanoates Synthesis Technology in Pilot Production Conditions