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In Ginger, it is necessary to find an alternative method for production of disease free planting materials as the conventional propagation technique through rhizomes transmit many diseases. The present study summarizes an efficient micropropagation method developed for Zingiber officinale Rosc. (Ginger) cultivar, Local, Chinese and Rangun using sprouted bud explants from fresh rhizomes. Newly sprouted rhizome buds with the size of 0.5-1.0 cm were surface sterilized with 30% Clorox for 30 minutes prior to culture and this treatment resulted 60%-70% healthy pure explants for all three cultivars. Murashige and Skoog (1962) medium has shown the best performance in culture establishment for three cultivars. Highest survival rate of the explants was observed in the cultivar, Chinese while lowest was observed in Local ginger. MS medium supplemented with 2mgl BA and 0.25 mg l NAA was shown the best performance for initial multiplication of ginger buds (8.6 shoots per explants) in the cultivar, Local. However buds of Rangun and Chinese were shown the highest rate of multiplication (respectively 7.33 and 7.20 shoots per explants) in the MS supplemented with 4.0 mg l BAP and 0.25 mg l NAA. Half strength of MS medium supplemented with 1.5 -2.0 mg l NAA has shown induction of roots in ginger cultivars tested. Of the combination tested 1⁄2 strength MS with 1.50 mg l NAA elicited optimal rooting response in which an average of 5.5 roots with 3.64 cm. root length for cultivar Local. But highest value of root length 4.95 (cm) and 4.12 (cm) were recorded in plantlets of Rangun and Chinese respectively in the half strength MS medium with 2.0 mgl NAA. Further experiments are being continued for acclimatization of in-vitro raised plants.

IN-VITRO MULTIPLICATION OF GINGER (Zingiber officinale ROSC.) CULTIVARS LOCAL, CHINESE AND RANGUN