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In my research, I studied the antioxidant effect of extracts of various rosemary samples obtained by Soxlet extraction on MCF-7 epithelial cells. I wanted to discover which of the extracts will have the highest content of total phenols determined in vitro with the Folin-Ciocalte reagent. The results show the highest content of total phenols in the rosemary sample from North Macedonia, so I assumed that it would have the biggest effective activity on MCF-7 cells. While doing the free radical inhibition test, I came to the conclusion that the plant with the highest antioxidant content does not necessarily have the best effect on the cells at different concentrations of the extracts. I suppose that it happens because antioxidants are polar and they need to transport through cell membrane conveyors whose number is limited in the membrane, that is, more antioxidants get assembled on the conveyors and cannot go through the membrane.

The Influence of Concentration of the Antioxidants in Rosemary Extract on MCF-7 Cells