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Effect of commercial cellulose enzymes was investigated by batch enzymatic hydrolysis at 15.0% (w/v) solid. It was found that the best commercial cellulose enzyme was Cellic CTec comparing to Accellerase 1000 and Accelerase 1500. The Cellic CTec gave the highest reducing sugar concentration and rice straw conversion. Moreover, when the hydrolysate obtained from hydrolysis using Cellic CTec was fermented by Saccharomyces cerevisiae TISTR 5596, it would give the highest ethanol. In this study, the Cellic CTec was used for fed-batch prehydrolysis prior to ethanol production by simultaneous saccharification and fermentation (SSF) way at 20% (w/v) solid loading. It could produce 35.76 g/L or 4.6% (v/v) of ethanol concentration and 83.67 L/ton dry matter (DM) of yield.

Effect of Commercial Cellulase Enzymes on Ethanol Production from Pretreated Rice Straw at High Solid Loading