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The main aim of this study was to investigate experimentally the effect of different composition of non-structural carbohydrates (NFC) in prepartum feed rations administered to high-yielding dairy cows at a high concentration of NFC in the diet on dry matter intake both before and after parturition and on subsequent milk performance, body condition and physiological traits of rumen fluid and blood. Thirty-six high-yielding dairy cows were allocated into one of the three well-balanced groups (K, O, and C), and each group received a different feeding rations. Feeding rations differed in non-structural carbohydrate (NFC) structure. The "K" (control) group received a feeding ration with NFC in the form of maize starch in particular, while the feeding rations of the other two (experimental) groups contained either (besides maize starch) saccharose from dried sugar beet (the "O" group) or a dominant amount of NFC was in the form of saccharose (the "C" group). After calving, all dairy cows were given the same feeding ration from the first day after parturition. The experiment was conducted for 21 days before and 50 days after calving. FR in the form of total mixed ration was offered ad libitum. Dry matter intake, milk performance, body condition, live weight, and blood and rumen parameters were recorded for the duration of the experiment. Average daily dry matter intake before calving was highest in the "K" group (14.32 kg per head). Differences among groups were statistically significant (P &amp;lt; 0.05). Prepartum dry matter consumption dropped as the rate of saccharose in the diet of cows increased. Dry matter consumption levelled off after calving. Milk yield was also highest in the "K" group (43.71 kg/head/day), but fatness of milk and thus the production of fat corrected milk were lowest in this group. The highest milk fat content (4.10%) and fat corrected milk production (44.03 kg/head/day) were recorded in the "C" group, whereas the highest milk protein concentration was found in the milk of the "O" group. The composition of NFC affected dry matter intake before parturition, but these concentrations did not significantly affect dry matter intake, milk yield, milk composition, live weight, body condition or blood serum and rumen fluid parameters after calving

Non-structural carbohydrates in the nutrition of high-yielding dairy cows during a transition period