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space management involves measures aimed at the protection of soil organic matter (SOM). In the EU countries, this issue is regarded as a priority, which was expressed in the proposal for the Directive of the European Parliament and of the Council establishing a framework for the protection of soil. Decreasing content of SOM in soils and the risks associated with its mineralization, besides lowering the productivity of soils (Seremesic et al. 2017), have environmental significance, leading to high emissions of carbon dioxide from soils (Jäger et al. 2011, Šimková et al. 2014). The formation of SOM is supported by fertilization, in particular with organic fertilizers originating from animal production. The effect of slurry depends on its chemical composition, dry matter content, the time of application and the dose. It is therefore necessary to monitor the effects of the use of slurry, in particular where it has been applied for several years during the time of experiment on the same field. The SOM balance in soils fertilized with slurry is determined by the dose applied and, where it is balanced in terms of the amount of carbon introduced with the commonly used dose of manure, its humus-forming value may equal 90–97% in relation to manure (Sądej and Namiotko 2011). In terms of the fertility of soils and the assessment of soil degradation, not only SOM but also the quality of the humic compounds being formed is important (Doni et al. 2014). As regards manure, an increase is observed in both the loosely and tightly bound fractions of the soil humus (Yan et al. 2007). The regular use of manure increases the content of the fraction of humic acids carbon (CHA), especially the fraction bound with calcium (CHA-Ca), fulvic acids carbon (CFA) and the humin fraction (Lapa et al. Comparison of the effect of various long-term fertilization systems on the content and fractional composition of humic compounds in Lessive soil