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Deligöz A., Bayar E., Genç M., Karatepe Y., Kırdar E., Cankara F.G. (2018): Seasonal and needle age-related variations in the biochemical characteristics of Pinus nigra subsp. pallasiana (Lamb.) Holmboe. J. For. Sci., 64: 379–386. Variations in the photosynthetic pigments and total carbohydrate contents of needles of different age classes (currentyear, 1-year-old, 2-year-old and 3-year-old) of Pinus nigra subsp. pallasiana (Lambert) Holmboe trees in a young natural stand were investigated during the growing season. In current-year needles, total carbohydrate content was lower during June and July when the needle growth continued than in older age classes, but it was similar to other age classes in the months of August to October. Seasonal patterns of variations in total carbohydrate content were almost similar in 1-, 2-, and 3-year-old needles. Chlorophyll and carotenoid contents increased from May to June, remained relatively constant or declined slightly during summer and autumn in 1-, 2-, and 3-year-old needles. In October, the pigment content was highest in 1-year-old needles, and lowest in 3-year-old needles. Our study indicated that total carbohydrate and pigment contents were affected by needle age classes and seasons.