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Sera of 204 wild boars (Sus scrofa), shot by hunters in the South-Moravian district of Břeclav during 1993- 2001, were tested by microagglutination reaction using safranin-stained antigens of Francisella tularensis and Brucella abortus: 10.8% and 8.7% seroreactors, respectively, were detected. The highest (17%) prevalence of tularaemia antibodies was found in wild boars during 1993-1994 at the beginning of a widespread outbreak of tularaemia in South Moravia that started in 1994, a nonsignificantly lower (13%) seroprevalence in 1995-1996 during the continuing epizootic, whereas it decreased markedly to 3% in the years 1997-2001 during the disappearance of the epizootic. Brucella sp. antibodies were significantly most frequent (15%) in wild boars in the years 1995-1996. This Brucella seroreactivity has been attributed to B. suis biotype 2 (B. melitensis biovar Suis biotype 2 according to new nomenclature) infection, because B. abortus in both cattle and humans (Bang's disease) was eradicated in the former Czechoslovakia in 1964. The hare brucellosis (B. suis bio- type 2) has occurred in the Břeclav district in a number of natural foci revealing an increased activity since 1994.

Serological survey of the wild boar (Sus scrofa) for tularaemia and brucellosis in South Moravia, Czech Republic