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in conifers and other plants with long reproductive cycles, transformed embryogenic tissues can serve as a convenient source of plant material for the testing of insecticidal or fungicidal transgene efficiency. i n this report, transgenic embryogenic tissue was obtained after the transformation of somatic embryos of norway spruce (Picea abies (l.) Karst.) by Agrobacterium tumefaciens with the gus -intron chimeric gene. t he stable integration of transgenes was confirmed by P cr and s outhern hybridization. t he transformation was successful only in a suitable embryogenic cell line sensitive to Agrobacterium. out of the nine embryogenic lines tested only one gave transgenic callus.

Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants