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Šařec P., Novák P. (2017): Changes in soil properties due to the application of activators in conditions of very heavy soils. Res. Agr. Eng., 63 (Special Issue): S40–S45. This paper deals with verification of the effect of fermented manure (with and without Z'fix activators) and soil activators (PRP Sol) on a soil properties change. Their application should lead to a change in physical, physical-chemical and biological properties of soil, along with ecological material fixation, improved water retention and infiltration, reduction of soil susceptibility to water erosion and decreased soil tillage energy requirements. Field trial was established in Sloveč in Central Bohemia in the year 2014. The experiment was divided into several variants and was designed as multiannual. Z›fix activator was used as a biological transformation activator of manure. PRP Sol was used as a soil activator. In order to verify the effect, soil infiltration, cone index, bulk density and draft of tillage implement were measured. Measurements have shown a beneficial effect of the activators with regard to the decomposition of organic matter. Consequently, changes in soil properties and a reduction of draft at tillage operations developed. Finally, the effect should evolve gradually with a prolonged activator treatment.