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CAPS (Cleaved Amplified Polymorphic Sequence) method and standard infection tests were used for the study of tomatoes (Lycopersicon esculentum L.) resistance against the root-knot nematode (Meloidogyne incognita Kofoit and White). The CAPS method was used to determine genotypic constitution of M i gene in ten Czech and four foreign varieties of tomatoes. Similarly were verified one hundred and twenty individuals of F 2 segregated progeny of Nema variety and the same number of F 2 progeny of Petopride variety. A comparison of results of infection test with results of molecular-genetic analyse of DNA showed 100% congruence in detection resistant (susceptible respectively) genotypes. Result of χ 2 test confirmed in the level of mathematical expectation P ∈ (0.5, 0.7) congruence between actually obtained and theoretical segregation ratios. The CAPS method can be recommended for fast and precise determination of resistance or susceptibility of tomato plants against the root-knot nematodes. The goal of the method is the possibility of detection of genotypic constitution of M i gene that can significantly accelerate the process of creation of new resistant varieties.

Using DNA markers for characterisation of tomato resistance against root nematoden Meloidogyne incognita