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Dlabaja T., Malaťak J., 2013. Optimization of anaerobic fermentation of kitchen waste . Res. Agr. Eng., 59: 1–8. Anaerobic fermentation is a suitable method of energetic and material utilisation of waste coming from restaurants and canteens. Laboratory experiments of wet anaerobic fermentation were performed in a continuous reactor and in batch reactors under mesophilic conditions. Effects of hydraulic retention time, organic loading rate, period of feeding and recirculation of digestate were examined in the continuous reactor. Effects of substrate pre-treatment (crushing, heating, freezing) were examined in the batch reactors. Degree of substrate degradation ranged between 83–85% within hydraulic retention time of 2–30 days. Appropriate organic loading rate was found in the range 2–3 kg of volatile solids per m 3 /day. Recirculation of digestate (both an inoculum for fresh substrate and replacement of fresh water supply) caused an increase in ammonia concentration and led to immediate inhibition of the process. The results further showed a positive effect of substrate pre-treatment in the initial phase of fermentation. However, degree of degradation did not show a significant increase for the period of 20 days of fermentation.