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Drazic G., Milovanovic J., Ikanovic J., Petric I. (2017): Influence of fertilization on Miscanthus × giganteus (Greef et Deu) yield and biomass traits in three experiments in Serbia. Plant Soil Environ., 63: 189–193. Miscanthus × giganteus (Greef et Deu) is an agro-energy crop of the second generation cultivated in purpose to obtain annually renewable bio-fuel produced from the aboveground biomass. Cultivation is preferred on marginal lands to avoid occupation of arable lands. Influence of fertilization and soil type Gleysol, Planosol and Technosol (open pit coal mine overburden) on yield and biomass traits of miscanthus were investigated during five years’ field experiment. Among biometric characteristics: stem height, length and width of leaves, the number of leaves (dry and green) per stem and number of stems per rhizome, only the last one has a strong positive correlation with yield. Fertilization increased yield during fourth and fifth year of development on Gleysol and Technosol. The highest yield on Gleysol was 23.12 t/ha in 2014, on Planosol 10.16 t/ha, and 4.77 t/ha in 2015 on Technosol. The yield of miscanthus, beside fertilization, depends on weather conditions and weeds. Cultivation of miscanthus is possible on marginal soils with minimum application of agricultural measures only in the year of establishment. Gleysol is a type of soil that can be recommended for miscanthus cultivation.