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To evaluate the effects of nano priming on seed germination of Alnus subcordata (Caucasian alder) C.A. von Meyer under drought stress, the present research was conducted using multi-walled carbon nanotubes (MWCNTs; 0, 10, 30, 50, and 100 mg·l–1) at 6 levels of drought stress (0, –2, –4, –6, –8, and –10 bar) through a factorial experiment (5 priming levels with 4 replications). After priming, the seeds were placed into a germinator at 21°C. Results revealed that nano priming at the concentration of 100 mg·l–1 led to the highest germination rate and percentage at all levels of drought stress. Also, the highest values of seed vigour index and root and stem lengths and dry weights were observed at nano carbon treatment with 30 mg·l–1. Considering the obtained results, it was concluded that nano priming could result in boosted resistance of Caucasian alder seeds against drought stress, so that the seed tolerance increased from –4 bar (without nano priming treatment, i.e. reference sample) to –8 bar upon applying 100 mg·l–1 nanotubes. Based on the results of the present research, it is suggested that the seed nano priming technique with MWCNTs can be applied in order to increase the seed and seedling tolerance of other members of the genus Alnus Miller.

Increasing drought resistance of Alnus subcordata C.A. Mey. seeds using a nano priming technique with multi-walled carbon nanotubes