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We conducted two laboratory experiments to examine the eectsoffertilisationandagitation(aeration)oncrudeoil degradation in two soils with dierentialnutrient(nitrogen,phosphorus)availability.Twosoilsthathadbeenspiked with crude oil two years before were mixed with nitrogen and/or phosphorus at three dierentlevelsandsubsequently incubated 28 days (Exp. 1). In experiment 2 we investigated the eectofrepeatedagitation(manualmixing) on hydrocarbon degradation with and without fertilisation. One of the soils was also freshly spiked to as-sess the impact of ageing. Heptane-extractable hydrocarbon concentrations were determined in both experiments and substrate-induced respiration in Exp. 2. Thegenerallysmallchangesofhydrocarbonconcentrationsduring28days of incubation in Exp. 1 are likely attributed to low bio-accessibility of hydrocarbons as a consequence of long-term ageing. Fertilization of nitrogen, phosphorus or combinations thereof was ineectiveinmosttreatmentsofExp. 1, which may be explained by limited oxygen supply due to the high proportions of clay and silt. However, agi-tation enhanced HC biodegradation in the sandy-loamy soil by about 15% (Exp. 2) compared to non-agitated treat-ments. In contrast, we observed no eectofagitationinthesandysoil.Keywords: biodegradation; crude oil; hydrocarbon; fertiliser; contaminated soils; incubation

Effect of N and P fertilisation and aeration on biodegradation of crude oil in aged hydrocarbon contaminated soils