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The expression of a PGIP gene (polygalacturonase inhibitor protein) was monitored with semi-quantitative (SQ)-RT-PCR in green, white and red berries of the strawberry cultivar Korona upon infection with Botrytis cinerea and wounding. In addition, the PGIP expression in infected white berries was quantified in four additional cultivars. The constitutive expression of PGIP increased from green to red berries in Korona suggesting developmental regulation of the gene. Wounding and fungal infection caused a moderate or a high induction in the PGIP level, respectively. The maximum peak was observed 24 h after the treatments. In the comparative experiment with five cultivars, infection of white berries caused an induction in the PGIP level 24 h after inoculation in four out of five cultivars.

Temporal expression of a PGIP-gene in strawberry cultivars induced by wounding or by Botrytis cinerea infection