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Control of SVBV relies completely on the use of virus-free planting material, that can be tested either by biological indexing or by molecular methods. A NASBA-based amplification was developed for the detection of SVBV. NASBA is a method based on the primer-dependent, specific amplification of RNA by concurrent activity of a special enzyme mix (AMV-reverse transcriptase, RNaseH, T7 RNA polymerase) at a single temperature (41°C). Specific and sensitive detection of the amplified sequence can be performed in the same tube using molecular beacons. Sensitivity of SVBVNASBA was 102 molecules of in vitro RNA detected per reaction. Results of the NASBA-based detection of SVBV in indicator strawberry plants were well comparable to the results of PCR.

plant protection science

Improved diagnostic tools for the certification of strawberry propagation material - the use of PCR and NASBA for detection of Strawberry vein banding virus (SVBV)