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Cryopreservation of sperm cells is an essential process applied for long-term conservation of aquatic genetic resources. The goal of this research was to determine effect different of straws volumes and thawing rates on the post-thaw quality and fertilization ability of cryopreserved common carp ( Cyprinus carpio ) sperm. In this study, semen was cryopreserved according to conventional slow freezing protocol. For this aim, the cryosolution contained 75 mM NaCl, 70 mM KCl, 2 mM CaCl 2 , 1 mM MgSO 4 and 20 mM Tris (pH: 8) supplemented with 10% MeOH. Following equilibration at +4°C for 10 min, semen was packed into 0.25, 0.5 and 1.5 mL straws and frozen in liquid nitrogen vapour (for 10 min at -120oC) and finally stored in liquid nitrogen (-196oC) tank. Thawing of cryopreserved semen process was performed at 30oC for 10, 20 and 30 seconds in a water bath. Fertilization was performed using ratio of 1x10 5 spermatozoa/egg. The highest fertility (68.4±2.5%) was determined with cryopreserved sperm packed in 1.5 mL straws that thawed at 30oC for 30 s. According to the results of this research, sperm cryopreserved with ionic extender containing 10% methanol and packed in 1.5 mL straws are suitable to achieve high fertilization of common carp eggs.

journal of limnology and freshwater fisheries research

Effect of Different Straw Volumes and Thawing Rates on Post-Thaw Quality and Fertilization Ability of Cryopreserved Common Carp (Cyprinus carpio) Sperm