First report of the isolation of entomopathogenic nematode Steinernema australe (Rhabditida: Steinernematidae) from South Africa

FUNDING: National Research Foundation (South Africa), Gauteng Department of Agriculture and Rural Development, University of the Witwatersrand A survey was conducted in Walkerville, south of Johannesburg (Gauteng, South Africa) between 2012 and 2016 to ascertain the diversity of entomopathogenic nematodes in the area. Entomopathogenic nematodes are soil-dwelling microscopic worms with the ability to infect and kill insects, and thus serve as eco-friendly control agents for problem insects in agriculture. Steinernematids were recovered in 1 out of 80 soil samples from uncultivated grassland; soil was characterised as loamy. The entomopathogenic nematodes were identified using molecular and morphological techniques. The isolate was identified as Steinernema australe. This report is the first of Steinernema australe in South Africa. S. australe was first isolated worldwide from a soil sample obtained from the beach on Isla Magdalena – an island in the Pacific Ocean, 2 km from mainland Chile.