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Ferritin is present in all cells of an organism, present in plasma and serum and considered a reflection of iron stores. Ferritin can be measured using enzyme linked immune sorbent assay (ELISA), radioimmunoassays (RIA), immunoradiometric assays (IRMA), agglutination methods and chemiluminescent methods. The i-CHROMATM Ferritin is a point of care test (POCT) lateral flow chromatography, fluorescence immunoassay (FIA) for the quantitative determination of ferritin in serum or plasma. In this study, the performance of the i-CHROMATM Ferritin method was evaluated with other laboratory ferritin methods using Cycle 41 from the Randox International Quality Assessment Service (RIQAS). The i-CHROMATM Ferritin correlated well with other laboratory ferritin methods: Abbott Axsym, Abbott Architect Chemiluminescence, Beckman AU400/600/640/2700/5400, bioMerieux, VIDAS, Roche Cobas C501/502/701, Roche Cobas E601/602, Siemens Centaur CP, Siemens Centaur XP/XPT/Classic, Siemens Dimension, Siemens/DPC Immulite 2000/2500, Siemens/DPC Immulite 1000, Beckman DxI 600/800, Roche Integra, DiaSorin, Liaison, Monobind Inc. ELISA, Roche Cobas 4000/c311, Roche Modular E170/Cobas e601/e602, Beckman, Access/LXi725 and Ortho Vitros 3600/5600/ECi. The results of this comparative study have shown that the i-CHROMATM Ferritin estimations were consistently lower ranging between +6% to -125%, but there was very good linear regression and correlation. The laboratory methods that were closest to the estimations of the i-CHROMATM Ferritin method were the Monobind Inc ELISA(Y=1.1082X 31.819; r2=0.9571), Ortho Vitro 3600/5600/ECi(Y=0.9805X-21.052; r2=0.9346) and the Beckman Dxl 600/800(Y=0.9012X-16.848; r2=0.9171). In summary, the i-CHROMATM Ferritin method correlated well with other laboratory methods, however, there was a significant negative bias seen. Therefore, it is important to take these negative biases into consideration when applying reference values.

The Performance of the Point of Care Test (POCT) i-CHROMA Ferritin Method and other Methods Enrolled in the RIQAS