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. The properties and functions of γ-aminobutyric acid (GABA A ) receptors in the mammalian central nervous system are well studied. However, the presence and significance of GABA A  receptors in nonneural tissue is less clear. The goal of this study was to examine the expression and localization of the GABA A  receptor β 2  and β 3  subunits in the kidney. Reverse transcriptase products from RNA isolated from rat and rabbit kidney cortex and cerebellum and rabbit S 2  segments were amplified by use of PCR and GABA A  β 2  and β 3  subunit—specific primers. Sequencing of the kidney PCR products revealed that the rat kidney cortex and rat neuronal GABA A  receptor β 2  subunit were identical in nucleotide composition. The rabbit kidney and rabbit neuronal GABA A  receptor β 2  subunit were 99% identical in nucleotide composition. Sequencing of the kidney PCR products revealed that the rat kidney cortex and rat neuronal GABA A  receptor β 3  subunits were 93% and 95% identical in nucleotide and amino acid composition, and rabbit kidney cortex and rabbit neuronal GABA A  receptor β 3  subunits were 95% and 98% identical in nucleotide and amino acid composition, respectively. PCR screening of a human kidney cDNA library and sequencing revealed that the human kidney cortex and neuronal β 3  subunits were identical in nucleotide composition. Immunoblot analysis of rat kidney cortex and brain identified immunoreactive proteins in the 55 to 57 kD region, corresponding to the GABA A  receptor β 2  and β 3  subunits. Immunohistochemistry revealed cytosolic and basolateral staining of the proximal convoluted and straight tubule. These results provide compelling evidence for the expression of the GABA A  receptor β 2  and β 3  subunits in the kidney of multiple species and the localization of the β 2 /β 3  subunits to the renal proximal tubule.

Identification of the γ-Aminobutyric Acid Receptor β2 and β3 Subunits in Rat, Rabbit, and Human Kidneys