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The fine structural localization of osmium impregnation in the mouse adrenal cortical cell was studied using the electron microscope. After osmication for 40--48 hrs all kinds of adrenal cortical cells of the mouse exhibited a selective localization of osmium impregnation in almost all the cisternae of the smooth endoplasmic reticulum in addition to 1--2 or 3 stacks and vesicles of cis-side of the Golgi apparatus. This fact might be implicated with the steroid hormones which may be localized in the cisternae of smooth endoplasmic reticulum. Moreover, the cisternae of the nuclear envelope, and of the characteristic lamellar body closely related to the smooth endoplasmic reticulum, were also stained with OsO4.

Characteristic reaction of the mouse adrenal cortical cell to osmium impregnation.