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Supplement of guanidine hydrochloride to the original tannic acid solution of MURAKAMI (1973) eliminated, without debilitating the conductivity effect, the vexatious treatment with different amino-acids and sucrose in the previous method (MURAKAMI, 1974). The procedures and advantages of this simplified modification are described using human spleen samples; attempt was made to elucidate the structure of arterial terminals by dissecting the specimens under the scanning electron microscope.

archivum histologicum japonicum

Modified Tannin-Osmium Conductive Staining Method for Non-Coated Scanning Electron Microscope Specimens. Its Application to Microdissection Scanning Electron Microscopy of the Spleen