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The distribution of NADPH-diaphorase positive cells was examined histochemically in the retinae of the pigeon and frog. In the pigeon, three different types of amacrine cells were identified in the inner nuclear layer (INL) on the basis of cell body size and staining intensity. In the frog two types of NADPH-diaphorase positive amacrine cells have been demonstrated in the INL. Therefore, the NADPH-diaphorase method selectively stains several subtypes of amacrine cells in the retina of lower vertebrates. Although the actual function of NADPH-diaphorase activity is unknown, diaphorase histochemistry provides a convenient method for achieving Golgi-like images of amacrine cells in the retina.

NADPH-diaphorase positive amacrine cells in the retinae of the frog (Rana esculenta) and pigeon (Columbia livia).