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Using a monoclonal antibody against human erythropoietin, its immunoreactivity was localized in the endothelial cells of peritubular blood vessels in the anemic mouse kidney. Other cell components of the kidney including glomeruli and tubules were negative. The non-anemic, normal kidney failed to reveal erythropoietin immunoreactivity.

Immunocytochemical demonstration of erythropoietin immunoreactivity in peritubular endothelial cells of the anemic mouse kidney.