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A new sensitive method has been established for light microscopic immunohistochemistry. It involves a protein A gold technique instead of immunoglobulin-gold method, followed by an improved procedure of physical development. In this procedure, two selected reagents were employed: bromohydroquinone, a more potent developing agent than hydroquinone, and specifically purified gum arabic solution. The results obtained by this method in the pancreatic islet tissues of the rat under a variety of histochemical control conditions have substantiated both the high specificity and fidelity of this method.

Protein A gold-silver staining method for light microscopic immunohistochemistry.