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The present study deals with an immunocytochemical localization of S-100 protein in the rat lymph nodes and spleen. The immunoreactivity for S-100 was contained in the reticular cells within the germinal center. The immunoreactive cells possessed a scanty perinuclear cytoplasm and extended slender cytoplasmic processes among lymphocytes. No cell outside the germinal center showed any immunoreactivity to the anti-S-100 serum.

S-100 protein-immunoreactive cells in the lymph node and spleen of the rat.