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caused by SINV-3 over the ensuing 3 years. We suspected that infectious SINV-3 particles were being disseminated in pieces of dried, friable ant corpses. Unlike SINV-1 and -2, which are limited to the midgut, the SINV-3 infection is systemic (Valles 2012). The extent of this suspected contamination was verified by conducting swab tests of the insectary for the presence of SINV-3 by RT-PCR. Surfaces of the floors and table tops in all 3 rearing areas, each in separate buildings at CMAVE were found contaminated with SINV-3 (mean 83%, n = 15). Indeed, dead ants in colonies often contained greater than 1 × 10 9 SINV-3

Procedures to Mitigate the Impact of Solenopsis Invicta Virus 3 In Fire Ant (Hymenoptera: Formicidae) Rearing Facilities