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The fall armyworm (FAW), Spodoptera frugiperda (J. E. Smith), is a polyphagous migratory pest, which is endemic to the Western Hemisphere attacking more than 80 plant species including maize, sorghum, cotton, rice, millet, peanut, alfalfa, and other cultivated and wild plant species (Knipling 1980; Pashley 1986; Vickery 1929). Presence of multiple generations and the ability to migrate and feed on a wide range of host plants makes FAW one of the most severe economic pests throughout the Western Hemisphere. In corn, yield losses due to FAW damage can reach up to 32% in the United States (Wiseman & Isenhour 1993) and 45-60% in Nicaragua (Hruska & Glandstone 1988). Insecticides are used as major components of IPM to control the pest, because its ability to migrate long distances and feed on a broad host range make other control options less efficient. Although chemical insecticides can provide effective control of crop pests including FAW (Young 1979), control of FAW has been fully dependent on insecticides, and, as a result, the pest has developed resistance to major classes of insecticides in several locations (Yu 1992; Berta et al. 2000; Pitre 1988). In Puerto Rico where many seed companies produce seed corn throughout the year, FAW is the number one pest, which makes it very difficult to get any yield without insecticide application. Insecticides are sprayed 3 times a wk during the peak season and up to 25 sprays have been made in one crop cycle, which places heavy selection pressure on the pest for development of insecticide resistance. The objective of the present study was to assess the susceptibility of Puerto Rico a FAW population to insecticides. A laboratory insecticide bioassay experiment was conducted at Dow AgroSciences (DAS) Research Station, Santa Isabel, Puerto Rico in Jun 2010. Third instar larvae derived from a field population of FAW collected from the central farm of the station on corn plants were used in a petri dish experiment. A total of 10 insecticides (Table 1) were evaluated each at the commercial (manufacturer’s) recommended rate. The insecticides were applied using small (200 mL) plastic hand sprayers. Two “shots” of fine droplets of the spray mixture were applied to each petri dish, which provided adequate coverage of the filter paper to mimic the field spray coverage. The untreated control larvae were sprayed with an equal amount of water to avoid the effect of moisture differences in the petri dishes. Treatments were replicated 5 times in a completely randomized design. Four insecticides i.e. spinosad, Spinetoram, acephate, and thiodicarb, which showed highest 16 h mortality of FAW in the first bioassay experiment, were selected and used in an additional study to evaluate dose response. Each of these insecticide was tested at 2 ×, 1 ×, 3⁄4 × and 1⁄2 × the recommended rate. The experimental set up and protocols were the same as in the first experiment. At 16 h after application, spinetoram, acephate, and thiodicarb caused significantly higher (≥60%) FAW mortality compared to other

Susceptibility of the Fall Armyworm,Spodoptera frugiperda(Lepidoptera: Noctuidae), at Santa Isabel, Puerto Rico, to Different Insecticides