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FTA® technology is an approach designed to expedite and simplify the collection, preserva tion, storage, shipment, and recovery of nucleic acid from the storage matrix (Whatman Inc. 2002a). FTA® cards have been used and evalu ated for DNA archiving of various biological sources (Moscoso et al. 2004; Harvey 2005). However, this method is expensive ($1.14/sam ple) and preparations can be elaborate, thus pushing researchers to devise alternative and cheaper methods for DNA preservation and storage (Owens & Szalanski 2005). Further more, in some cases, DNA retrieval from the FTA® cards is complex and requires highly se lective conditions (Hide et al. 2003; Adams et al. 2008). Hence, some researchers have had to modify the protocols to retrieve DNA from the FTA® cards in their studies (Silva et al. 2004; Smith & Burgoyne 2004). For termites, we have failed to obtain success ful amplifications from the nucleic acid processed using any of the manufacturer-recommended pro cedures. Thus, we have developed a new combina tion of methods for DNA recovery from FTA® cards.

An Improved Method for Extraction and Purification of Termite Endo-β-1,4-Glucanase from FTA® Cards