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The "annual bluegrass weevil", Listronotus maculicollis Kirby (Cole?ptera: Curculionidae) is a serious pest of Poa annua L. (Poales: Poaceae) turfgrass in the Northeastern U.S. Historically, the primary control strategy has been to intercept adults with insecticide residues as they emerge from overwintering sites (Cowles et al. 2008; Pot ter 1998; Vittum et al. 1999). Chlorinated hydro carbons were used to manage weevil populations until around 1969, but in the 1970s and 1980s less persistent organophosphates were utilized; today pyrethroids and chlorpyrifos are used to control adults (Cameron & Johnson 1971; Kop penh?fer & McGraw 2005; Schread 1970; Tashiro 1976). In 2009 several adult L. maculicollis popula tions from Connecticut, Massachusetts, and Rhode Island demonstrated varying levels of re sistance to pyrethroids (Ramoutar et al. 2009ab), but no data are available for L. maculicollis resis tance to chlorpyrifos, an organophosphate. Tash iro (1976) first communicated diminished organo phosphate effectiveness, but there are no further studies conducted on this topic. The objective of this study was to obtain toxicity data on the sus ceptibility of L. maculicollis populations from sev eral golf courses in southern New England to chlorpyrifos. In 2008 and 2009 from May-Sep adult wee vils were collected from 8 golf courses in Con necticut, Massachusetts, and Rhode Island (Fig. 1). Insects were collected by hand from golf course fairways or greens and kept at 21-23?C on P. annua plugs until bioassays were con ducted, within 48 h of collection. Chlorpyrifos (technical, 99.9% purity) was dissolved in re agent quality acetone (>95% purity) (Sigma-Al drich, St. Louis, MO) and 1 of chlorpyrifos or acetone (control) was applied per insect dor sally to the intersegmental membrane between the prothorax and the elytra (Metcalf 1958; Perez-Mendoza 1999). Mortality data were esti mated from 6-8 concentrations and 10-15 un sexed adults per concentration. Applications

Susceptability ofListronotus maculicollis(Coleoptera: Curculionidae) Adults from Southern New England Golf Courses to Chlorpyrifos