Open AccessElectroporation of Murine Embryonic Stem Cells: A Step‐by‐Step Guide
Author(s) -
Tompers Dennie M.,
Labosky Patricia A.
Publication year - 2004
Publication title -
stem cells
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.159
H-Index - 229
eISSN - 1549-4918
pISSN - 1066-5099
DOI - 10.1634/stemcells.22-3-243
Subject(s) - biology , embryonic stem cell , electroporation , stem cell , microbiology and biotechnology , homologous recombination , genetics , dna , gene
The manipulation of embryonic stem (ES) cells to generate targeted mutations via homologous recombination has proved an invaluable resource for researchers from fields as diverse as embryology, immunology, physiology, and biophysics. The derivation and culture of these cells has been described in many places, but there is no substitute for hands‐on experience. In lieu of that, here we provide a step‐by‐step guide for the technique of electroporation, complete with micrographs of the cells at each step of the process. This is intended to allow the first time ES cell worker, as well as the more experienced researcher, to view the morphology of ES cells under ideal conditions as well as when the cells are suboptimal and should be discarded.